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[Solved] BT503 Assignment 2 Spring 2020  


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29/07/2020 9:41 pm  

BT503 Environment Biotechnology Assignment 2 Solution & Discussion Spring 2020

BT503 Assignment 2 Solution idea:

Credit: Amaan Khan


Assignment No. 2

BT503–Environment Biotechnology

Spring 2020

Total Marks: 10   Due date: 04/08/2020

Assignment topic

Q: Write down the factors affecting the use of Bio-remediation. (10 Marks)

Bio-remediation is a technique which is used to remove the toxic chemical or pollutant from the environment by using the microorganisms. The microorganism helps to degrade the toxic material of the environment. So in this technique the microorganisms are very importance and every microorganism has specific condition for their survival which included different factors which are discuss below:

Factors affecting the use of Bio-remediation:


pH of the soil is important, because survival of most microbial species is limited to a certain pH range. In addition to soil pH can affect availability of nutrients present in the soil. Every microorganisms have a specific narrow range pH is known optimum pH.

Moisture content:

All soil microorganisms require moisture for cell growth and function. Availability of water affects circulation of water and soluble nutrients into and out of microorganism cells. However, excess moisture, such as in saturated soil is undesirable, because it reduces the amount of available oxygen for aerobic respiration. The force of attraction between water in the soil.


Temperature influences rate of biodegradation by controlling rate of enzymatic reactions within microorganisms. Generally the rate of enzymatic reactions in the cell about doubles for each 10 degree Celsius rise in temperature. There is an upper limit to the temperature that microorganisms can withstand. Most bacteria found in soil, including many bacteria that degrade petroleum hydrocarbons, are mesophiles which have an optimum temperature ranging from 25 degree C to 45 degree C .Thermophilic bacteria which are normally found in hot springs and exist indigenously in cool soil environments and can be activated to degrade hydrocarbons with an increase in temperature to 60 degree C.

Nutrient availability:

Inorganic nutrients such as nitrogen and phosphorus are necessary for microbial activity and cell growth. It has been shown that treating petroleum-contaminated soil with nitrogen can increase cell growth rate, decrease the microbial lag phase that helps to maintain microbial populations at high activity levels. It increases the rate of hydrocarbon degradation. Nevertheless, it has also been revealed that extreme amounts of nitrogen in soil cause microbial inhibition.

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